Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

Karen L. A. Guimarães^{1, 2} , Juan J. Rosso^{3, 4}  Mendelsohn F. B. Souza² Juan M. Díaz de Astarloa^{3, 4}  andLuís R. R. Rodrigues^{1, 2} 

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Abstract​

Introduction​

The wolf fish, locally named as “trahiras” in most part of South America, are classified in the family Erythrinidae, with 19 valid species and 3 genera: Hoplias Gill, 1903, Erythrinus Scopoli, 1777, and Hoplerythrinus Gill, 1896 (Fricke et al., 2020). These species have peculiar morphological features, such as, cylindrical body form, rounded caudal fin, absence of adipose fin, 8‒15 dorsal-fin rays, 10‒11 anal-fin rays, numerous teeth on the palate and 34‒47 lateral-line scales (Oyakawa, 2003). This family is restricted to Neotropical region, from Costa Rica to southern Ecuador in the west, and to Argentina in the southeast, being widespread in the South America freshwaters systems (Oyakawa, 2003; Berra, 2007).

Hoplias is the richest genus comprising 14 valid species (Fricke et al., 2020). Based on morphological features, the genus is arranged in three groups: Hoplias aimara (Valenciennes, 1847), Hoplias lacerdae Miranda Ribeiro, 1908 and Hoplias malabaricus (Bloch, 1794) (Oyakawa, 1990; Mattox et al., 2006; Oyakawa, Mattox, 2009). Recent efforts to try to solve the complex taxonomy of the Hoplias malabaricus group have contributed with the re-description (Mattox et al., 2014) and description of new species (Azpelicueta et al., 2015; Rosso et al., 2016, 2018). Currently, this group comprises six species: H. malabaricus, H. microlepis (Günther, 1864), H. teres (Valenciennes, 1847), H. mbigua Azpelicueta, Benítez, Aichino & Mendes, 2015, H. misionera Rosso, Mabragaña, González-Castro, Delpiani, Avigliano, Schenone & Díaz de Astarloa, 2016, and H. argentinensis Rosso, González-Castro, Bogan, Cardoso, Mabragaña, Delpiani & Díaz de Astarloa, 2018. The improvement in taxonomic discrimination intimately agrees with historical cytogenetic studies demonstrating that H. malabaricus is a species complex that hinders cryptic diversity (Bertollo et al., 1997, 2000; Born, Bertollo, 2006; Cioffi et al., 2009; Blanco et al., 2010; Da Rosa et al., 2014).

Just recently, molecular results revealed the existence of several fully supported lineages within the once considered to be the continentally distributed H. malabaricus (Cardoso et al., 2018; Jacobina et al., 2018). Many of these lineages were found in the Amazon basin, where Marques et al. (2013) earlier demonstrated a conspicuous genetic distinctiveness in a H. malabaricus population (Haplogroup Gp2). This population was tentatively assigned to H. misionera (Cardoso et al., 2018) as it shares the same molecular identity (BIN AAB1732) of the type material of this species. Nevertheless, a taxonomic revision of the Amazon population is lacking. The eventual occurrence of H. misionera in the Amazon basin would greatly expand the geographic distribution of this species, since, up to now, its distribution was restricted to the Uruguay, Paraná and Paraguay River Basins in Argentina and southern Brazil (Rosso et al., 2016).

Hoplias misionera is distinguished from congeners by the presence of Y-shaped configuration in the medial margin of dentaries, predorsal scales (15‒17), total vertebrae count (39‒40) and series of the last vertical scales on caudal fin forming a marked curve (Rosso et al., 2016). The evaluation of these characters as well as complementary molecular tools would certainly properly define the taxonomic status of the Amazon population postulated to be H. misionera. Indeed, modern integrative approaches combining morphological and molecular tools suggest that several divergent lineages may constitute fully independent species in Neotropical Teleosts (Pugedo et al., 2016; Rosso et al., 2018). Herein, we investigate the taxonomic status of a Hoplias population from the Amazon basin by means of an integrative approach including morphological, DNA barcoding and cytogenetic considerations.

Material and methods

Ethics statement. The Brazilian government System of Authorization and Information in Biodiversity (SISBIO) provided the permits for fish collections (SISBIO 32653–3). The animals were anesthetized and euthanized through immersion in water containing Eugenol solution, following a procedure approved by the Animal Use Ethics Committee (CEUA) of the Universidade Federal do Oeste do Pará (CEUA/UFOPA Nº 09003).

Sampling and study area. We analyzed 23 specimens collected during fieldwork in the lower Amazonas and Trombetas Rivers, in Pará State (Tab. 1). Three specimens from Viçosa Island, in the Marajó archipelago, were purchased in a fish market in the Macapá city, Amapá State.

Morphological analysis. Voucher specimens were fixed in 10% formalin during 72h, rinsed with tap water and preserved in 70% ethanol. Measurements and counts were taken on the left side of the body following Fink, Weitzman (1974), Mattox et al. (2006) and Rosso et al. (2018) protocols. Counts were obtained by either visual or microscopic inspection. Linear body measurements were taken with a digital caliper to the nearest 0.1 mm. Vertebral counts were obtained using radiographs and included the four anterior vertebrae of the Weber apparatus. The examined vouchers (n=9) are deposited in the Laboratório de Genética e Biodiversidade, Universidade Federal do Oeste do Pará (UFOPA), Brazil and listed herein in the section material examined. Abbreviation institutions: (UNMDP) Universidad Nacional de Mar del Plata, Argentina.

TABLE 1 | Detailed geographic information of new and former records of Hoplias misionera in Amazon Basin. N = number of specimens collected at each location.

Material examined (morphological data). Hoplias misionera: Brazil:Lower Amazonas River basin, UFOPA AMTRA126–19 – AMTRA128–19; UFOPA AMTRA 130–19 – AMTRA131–19, 5, 214–238 mm SL. Sapucuá Lake: UFOPA AMTRA037–11, 1, female, 188 mm SL. Marajó archipelago, Viçosa Island: UFOPA AMTRA122–19, AMTRA123–19, AMTRA125–19, 3, 228–262 mm SL.

Molecular analysis. Before fixation, a small muscle tissue sample from each specimen was collected and further preserved in absolute ethanol. Total genomic DNA was extracted following an adapted salting-out protocol (Aljanabi, Martinez, 1997; Vitorino et al., 2015). DNA barcoding sequences (COI mtDNA) were amplified by Polymerase Chain Reaction (PCR) using standard primers FishF1 and Fish R1 (Ward et al., 2005). Details of PCR profiles and sequencing reactions are given in Guimarães et al. (2018). In order to explore genetic divergences with already known species, we supplemented our COI data set with sequences downloaded from public repository Barcode of Life Database (www.boldsystems.org) (Ratnasingham, Hebert, 2007) of the following species: Hoplias misionera (n=48) (Marques et al., 2013; Rosso et al., 2016; Cardoso et al., 2018), H. malabaricus (n=10) (Marques et al., 2013; Cardoso et al., 2018), H. microlepis (n=7) (BOLD Systems), H. argentinensis (n=10) (Cardoso et al., 2018), H. mbigua (n=5) (Cardoso et al., 2018), and H. lacerdae (n=2) (Cardoso et al., 2018). Detailed information on DNA barcoding sequences and specimen origin are listed in S1.

The sequences were aligned using the ClustalW Algorithm (Thompson et al., 1994) implemented in the software BioEdit (Hall, 1999). Three species delimitation approaches were adopted to recognize Operational Taxonomic Units (OTUs): 1) Generalized Mixed Yule Coalescent – GMYC (Pons et al., 2006; Fujisawa, Barraclough 2013); 2) Automatic Barcode Gap Discovery – ABGD (Puillandre et al., 2012), and 3) Barcode Index Number (BIN) (Ratnasingham, Hebert, 2013).

For the GMYC method, the repeated haplotypes were removed and an ultrametric tree based on bayesian inference was constructed in BEAST v1.8.0 (Drummond, Rambaut, 2007) with the following settings: GTR evolution model (Gamma distribution), molecular clock lognormal relaxed and Yule process speciation. The bayesian reconstruction was ran with 100 million MCMC iterations, sampled each 1000 iterations with a burn-in of 10%. The convergence and stability were checked with the software Tracer v.1.7.1 and retained for Effective Sample Sizes (ESS) > 200 (Drummond, Rambaut, 2007). The resulting trees were combined with TreeAnotator v1.8.0 (Drummond, Rambaut, 2007). To test the branching events for speciation we used the packages Splits (Species Limits by Threshold Statistics) and Ape (Analyses of Phylogenetics and Evolution), with the single threshold model implemented in R 3.4.0 statistical software (R Core Team, 2014).

We used the method Automatic Barcode Gap Discovery (ABGD) to explore the existence of barcoding gap among the Hoplias taxa analyzed. This analysis was processed in the online platform (bioinfo.mnhn.fr/abi/public/abgd/abgdweb.html) and setting for K80 model, intraspecific divergence (min. 0.001 and max. 0.1) and barcoding gap as default (X=1.5). The Barcode Index Number (BIN) is implemented in the BOLD System workbench (www.boldsystems.org) that uses the algorithm RESL (Refined Single Linkage Analysis) to find OTUs of DNA barcodes from entry data and the BOLD archived library (Ratnasingham, Hebert, 2013).

For cluster visualization, a Neighbor-joining (NJ) tree based on Kimura-2-parameters (K2P) evolution model (Kimura, 1980) processed with the software MEGA X (Kumar et al., 2018) and edited with FigTree v.1.2.2 (http://tree.bio.ed.ac.uk/software/figtree/) was built. We estimated K2P genetic distances using MEGA X (Kumar et al., 2018). All the new DNA barcoding sequences were uploaded to the BOLD Systems database linked to the Project AMTRA: “Amazonian Trahiras”.

Cytogenetic analysis. We analyzed the karyotype of ten specimens (AMTRA003-11, AMTRA022-11, AMTRA023-11, AMTRA024-11, AMTRA025-11, AMTRA028-11, AMTRA029-11, AMTRA030-11, AMTRA031-11, AMTRA037-11). Chromosome preparations were obtained from kidney cells after 24h of yeast mitosis stimulation (Lee, Elder, 1980) and exposed to 0.025% colchicine (0.01ml/g body mass) following Bertollo et al. (1978). The metaphases were examined through 5% Giemsa conventional staining. C-banding followed Sumner (1972). We detected Nucleolar Organizing regions by silver staining (Ag-NOR) following Howell, Black (1980). In situ hybridization (FISH) was applied to mapping ribosomal genes DNAr 18S. The probes were done by PCR using the primers Forward: 18Sf (5’ CCG CTT TGG TGA CTC TTG AT 3’) and Reverse: 18Sr (5’ CCG AGG ACC TCA CTA AAC CA 3’) (Martins, Vicari, 2012). The FISH experiments followed procedures described in Pinkel et al. (1986) with minor adaptations as described in da Fonseca et al. (2018). The chromosomes in the karyotypes were visually classified as metacentrics (m) and submetacentrics (sm) based on arm ratio according to Levan et al. (1964) and arranged following Cioffi et al. (2009), Santos et al. (2009). In order to facilitate comparisons and karyomorph discrimination we analyzed the pattern in size reduction of the first four largest pairs, a criterion previously adopted to distinguish karyomorphs with same diploid number (Bertollo et al., 1997). In this comparative analysis we drawn partial idiograms using chromosome figs. from literature for H. malabaricus karyomorphs C and F (Bertollo et al., 1997), with assistance of the software Drawid V0.26 (Kirov et al., 2017).

Results​

Morphological analysis (identification). The external morphology and ethanol-preserved coloration are shown in Fig. 1. Medial margins of contralateral dentaries converged to midline in a characteristic Y-shaped (n= 4) or V-shaped (n= 5) (Figs. 2A,B) configuration. Basihial and basibranchials bones bearing tooth plates. A single premaxillary tooth row. First two premaxillary teeth large and caniniform, then four or five very small teeth followed by other two large canines. Maxilla with 35‒44 teeth, first five increasing progressively in size. Dentary external series comprised of 4 small teeth followed by two larger canines, then other series of 4‒6 small teeth and 8‒10 teeth arranged in a repetitive series of one large and one-two small conic teeth. Accessory ectopterygoids not fragmented, anteriorly expanded and bearing 12‒14 conical teeth along their ventrolateral margins. Total dorsal-fin rays 14‒15 (ii‒12 n = 2; ii‒13 n = 7). Total anal-fin rays 10‒11 (i‒9 n= 2; ii‒9 n = 7). Total pectoral-fin rays 13 (i‒12 n = 9). Tip of pectoral fin separated from pelvic-fin origin by 3‒5 scales. Total pelvic-fin rays 8 (i‒7 n = 9). Tip of pelvic fin separated from vertical through anus by 2‒3 scales. Total caudal-fin rays 17 (i‒15‒i n = 9). Predorsal scales (15‒17) arranged in an irregular series. Last vertical series of scales on caudal peduncle forming a curve (Fig. 3A). Lateral line complete with one or two anterior scales without pores followed by 39‒41 perforated scales. Longitudinal series of scales between dorsal-fin origin and lateral line 5‒5.5; between lateral line and pelvic-fin origin 4‒5. Longitudinal series of scales around caudal peduncle, invariable 20. First epibranchial with 9‒12 plate-like denticulated gill rakers. One laminar gill raker on cartilage. First ceratobranchial with 4‒6 more elongated rakers and 11‒16 plate-like denticulated gill rakers. Laterosensory canal along ventral surface of dentary with four pores. A single laterosensory canal along infraorbitals with invariable 11 pores, with infraorbital 5 lacking pores. Laterosensory system of dorsal surface of head with 11‒12 pores. Nasal bone: two pores, frontal bone: four-five pores, pterotic bone: two pores. One pore between parietal bones, on posterior end of symphysis. Supraopercle and extra-scapular bones with following combination of pores: 1:1 and 0:2. Total vertebrae 38‒40 (n=3).

FIGURE 1| Hoplias misionera, UFOPA AMTRA131-19, 237 mm SL, Amazonas River, Alenquer, Pará, Brazil. Lateral view. Scale bar = 1 cm. Photo by L.R.R. Rodrigues.

FIGURE 2| Configuration of the medial margins of the dentary in Hoplias misionera. A. Y-shaped, UFOPA AMTRA126-19, 214 mm SL. B. V-shaped, UFOPA AMTRA127-19, 232 mm SL. Scale bars = 1 cm. Photos by L. R. R. Rodrigues. Illustration by T. M. A. Lima.

FIGURE 3| Last vertical series of scales on the base of the caudal-fin rays. Comparison between Hoplias misionera (A), UFOPA AMTRA131-19, 237 mm SL and Hoplias cf. malabaricus (B), UFOPA AMTRA110, 201 mm SL. Scale bars = 1 cm. Photos by L. R. R. Rodrigues. Illustration by T. M. A. Lima.

Morphometric data of specimens of H. misionera from the Amazon River Basin are summarized in Tab. 2. The population of the Amazon basin was composed by specimens of 188 to 262 mm of standard length. A shallower body depth (18.1‒20.3% vs. 20.6‒25.5%), shorter head length (28.0‒30.9% vs. 30.6‒34.6%), smaller predorsal distance (42.4‒46.2% vs. 46.9‒51.8%) and larger snout length (24.0‒28.5% vs. 20.5‒24.7%) characterize this population when compared with H. misionera from La Plata River Basin.

Molecular analysis. A data set with 95 DNA partial COI sequences (652 bp) of six species of Hoplias was assembled and revealed a base composition of 17% G, 27.9% C, 23.8% A and 31.3% T. The sequences did not show indels nor stop codons. All the species delimiting tools were congruent to confirm the presence of H. misionera in the Amazon basin. The H. misionera cluster was either delimited in a single cluster (ABGD, BIN) or split in two clusters (GMYC). A tree inference based on distances (NJ) revealed clusters that were congruent with species delimitation (Fig. 4). All the specimens sampled to this work nested within the H. misionera clade. Pairwise genetic distances indicated deep divergences (4.7‒15.4%) between species. In contrast, H. misionera populations from Amazon basin and La Plata Basin diverged by just 0.6% (Tab. 3).

TABLE 2 | Morphometric data of Hoplias misionera from the Amazon basin; values 1-14 are percentages of the standard length and values 15-22 are percentages of head length. N = number of specimens; SD = standard deviation.

FIGURE 4| Neighbor joining (NJ) tree of Hoplias inferred from partial COI (Cytochrome c Oxidase Subunit I gene) sequences using the Kimura 2-parameter model. The lateral bar indicates the partitions of species delimitation performed by the GMYC, ABGD and BIN analysis. The clade Hoplias misionera nested individuals from the La Plata and Amazon basins (blue tips).

Cytogenetic analysis. The karyotype of H. misionera from Amazon basin presented 2n=40 chromosomes, where 20 pairs were metacentric and 20 submetacentric (Fig. 5A). C-banding showed heterochromatic regions in the centromeres of all chromosomes and variable amount in the distal region in most of the metacentric pairs (4‒10) and few submetacentric pairs (17‒20). A conspicuous heterochromatic block was observed in the pericentromeric region of pair 13 (Fig. 5B). The Nucleolar organizing regions (Ag-NORs) were detected in the telomeric region of two submetacentric pairs. These Ag-NOR positions were coincident with the 18S rDNA hybridization marks in the pairs 13 and 16; an additional fluorescent mark was visualized in the centromeric region of the metacentric pair 3 (Fig. 5C). The size and heterochromatic band comparison between the largest chromosome pairs (1‒2, 11‒12) are showed in Fig. 6.

TABLE 3 | Mean genetic distances (Kimura 2-parameter model) between Hoplias clusters. Bold values indicate the mean intraspecific distances.

FIGURE 5| Karyotype of Hoplias misionera from Amazon basin (2n=40 chromosomes). Conventional Giemsa stained (A), C-banded (B) and mapping of 18S rDNA FISH probes (green signals) (C). The Ag-NOR bearing chromosomes are showed in the box.

Geographic distribution. The easternmost collecting point in the Amazon basin, Viçosa Island, was situated in the Marajó Archipelago, a large island that lies at the mouth of the Amazon River. An updated distribution map of H. misionera is provided in the Fig. 7.

FIGURE 6| Partial idiogram of the four largest chromosome pairs of Hoplias malabaricus (karyomorphs C and F) and H. misionera showing marked size reduction from the first to second metacentric pair only in the karyomorph F. 

FIGURE 7| Updated distribution map of Hoplias misionera showing former known localities in Argentina and southern Brazil (Rosso et al., 2016) and the new records from Amazon basin (triangles). Star = type locality.

Discussion​

Our results combined morphology, DNA and cytogenetics to characterize a population of H. misionera from the Amazon basin and represent the first record of this species outside the La Plata River basin. The set of available diagnostic characters examined fits the diagnosis of H. misionera (Rosso et al., 2016), with remarks on some characters. Overall, when compared with type specimens from La Plata River basin, the population of H. misionera from the Amazon basin can be characterized by having a slightly higher number of total anal-fin rays (10‒11 vs. 10) and scales separating pelvic fin from anus (2‒3 vs. 2). They also presented lower number of teeth (8‒10 vs. 10‒16) in the posterior repetitive series of the dentary and scales in the lateral line (39‒41 vs. 40‒43). A slightly wider range was observed in counts of gill rakers in epibranchial (9‒12 vs. 10‒11) and ceratobranchial (15‒22 vs. 17‒21) bones.

Morphometric analysis revealed some trends of variation between Amazon and La Plata populations of H. misionera. The population of the Amazon basin was composed by larger specimens (188‒262 mm vs. 39.22‒174 mm of standard length) and showed different values ​​in body depth, head length, predorsal distance and snout length.

Morphological differences between natural populations of geographically isolated fish that inhabit different hydrographic systems have been frequently reported (e.g., Neves, Monteiro, 2003; Shibatta, Hoffmann, 2005; Silva et al., 2009). In particular, these studies propose that this external morphological distinction could be, in part, a response to selective pressure in different environmental conditions. Hoplias misionera is a species that inhabits different environments, such as rivers, streams, lakes and dams, where these morphometric differences may be the result of different evolutionary patterns due to environmental conditions, emphasizing the singularity of each basin. Some of the observed variable morphometric characters could also be the result of ontogenetic variation, since there was a complete non-overlapping range of standard length between both populations. The entire range of lateral-line scales (39‒43) combining Amazon and La Plata populations was observed by Ota et al. (2018) in the upper Paraná River basin. Reia et al. (2020) further expanded the lower limit to 38 when revising four specimens in the Sucurí River basin, upper Paraná River.

Some specimens of H. misionera from Amazon basin displayed a Y-shaped arrangement on the medial margins of dentaries, a feature firstly proposed to distinguish H. misionera from all remainder species of Hoplias (Rosso et al., 2016). However, we also observed the V-shaped configuration in some specimens, as it was also observed lately for other populations of H. misionera from the lower Paraná and Paraguay river basins (Rosso, unpublished data). Despite the variable state of this character, the remaining morphological and meristic characters analyzed in the specimens collected in the Amazon River basin were largely congruent with those provided by Rosso et al. (2016).

The molecular evidence also confirms that samples analyzed represent H. misionera. The speciation process within Hoplias has been linked to deep divergence in COI sequences. For instance, H. misionera and the recently described H. argentinensis diverged in 5.6 and 9.0% from the nearest neighbor (Rosso et al., 2016, 2018) respectively. In the Amazon basin, only one species of Hoplias malabaricus group (H. malabaricus) has been reported (see Oyakawa, 2003). Our results showed that Hoplias misionera of the Amazon basin presented 6.9% of genetic distance to this species. Generally, a 2% divergence threshold has been widely used to discriminate Neotropical fish species (Pereira et al., 2013), but there is also evidence that the interspecific genetic distance value of 2%, may not be useful in many Neotropical fish species, as occurs within the genera Astyanax Baird & Girard, 1854 (Rossini et al., 2016; Terán et al., 2020), Hypostomus Lacepède, 1803 (Queiroz et al., 2020), Schizodon Agassiz, 1829 (Ramirez et al., 2020), among others. Our molecular analysis revealed a low divergence between the two populations of H. misionera (Amazon vs. La Plata) diverging by just 0.5%. Indeed, inferences using COI gene showed that several taxonomically recognized species in the genus Hoplias form well defined mtDNA lineages (Cardoso et al., 2018). These results strongly suggest that barcode methodology should be considered as an additional diagnostic tool for confirmation of future new records for the genus Hoplias.

Hoplias misionera from the Amazonas River showed karyotype 2n=40 and its macrostructure resembles to the karyomorphs C and F of its congener H. malabaricus (Bertollo et al., 1997, 2000; Cioffi et al., 2009; Santos et al., 2009). Besides the conservative diploid number both karyomorphs are clearly distinguished based on the relative chromosomal size between the first four largest pairs (Bertollo et al., 1997) and some minor differences in the amount of constitutive heterochromatin that is slightly increased in the karyomorph C (see Cioffi, Bertollo, 2010; Santos et al., 2009, 2016). These attributes are assumed to validate both karyomorphs as distinct entities. Indeed, Bertollo et al. (1997) observed sympatry of karyomorphs C and F in the Tocantins River population without evidence of hybridization.

Our specimens showed karyotypic formulae (20m+20sm) similar to that observed in H. malabaricus karyomorph F from São Francisco river (Santos et al., 2009) and karyomorph C from Amazon basin (Marques et al., 2013; Santos et al., 2016). However, it differs from karyomorph C population from Bento Gomes River, a tributary of Paraguay River basin (Cioffi et al., 2009; Cioffi, Bertollo, 2010). In addition, H. misionera from Amazon basin shared identical pattern of Ag-NOR bearing chromosomes with H. malabaricus cytotype F and C from Amazon basin, but diverges from the Paraguay River basin population (see Cioffi et al., 2009; Cioffi, Bertollo, 2010).

It is noteworthy that karyomorph C is widespread throughout South America (Bertollo et al., 2000) and shows variation in some cytogenetic markers, such as karyotypic formulae, Ag-NOR and 18S FISH marks (Cioffi et al., 2009; Cioffi, Bertollo, 2010; Santos et al., 2016; Guimarães et al., 2017). Variation of karyotypic formulae is frequently explained by the occurrence of chromosomal rearrangements but sometimes it could be an artifact resulted from misinterpretation of chromosome morphology in poor metaphases plates. Given the good quality of Hoplias chromosome preparations is plausible that populations from distinct hydrographic basins, showing karyomorph C variants, can diverge by chromosomal rearrangements such as pericentric inversions, which is a good explanation for the transformation of 20m+20sm to 14m+26sm such as observed between H. malabaricus (karyomorph C) from Amazon and Paraguay river basins. It has been frequently demonstrated that H. malabaricus display multiple and variable Ag-NORs sites among distinct populations (Bertollo, 1996; Born, Bertollo, 2000; Vicari et al., 2005; Santos et al., 2016). Hoplias misionera conserved this cytogenomic feature (multiple Ag-NORs) and because this species shared a similar Ag-NOR pattern with karyomorphs C and F, is reasonable to conclude that based on this trait we cannot resolve its karyotype classification.

The relative size of the first four largest pairs has been considered a reliable trait to separate both karyomorphs C and F (Bertollo et al., 1997). The comparative analysis with the karyotype of H. misionera failed to detect the marked size reduction from the first to second chromosome pair, which is the main cytogenetic signature of the karyomorph F. In contrast, we observed a gradual size reduction congruent with the karyomorph C. Distinct populations of H. malabaricus karyomorph C share these same characters and additionally show high amounts of heterochromatin (Bertollo et al., 1997; Santos et al., 2016; Cioffi, Bertollo, 2010), a feature that was also clearly observed in our specimens of H. misionera. Additionally, the karyomorph C is characterized by a nascent XX/XY sex system that leads to heterochromatin accumulation in the centromere of pair 11 (Cioffi, Bertollo, 2010), homologue to pair 14 (Santos et al., 2016) and that we postulate homologue to the H. misionera pair 13. In contrast, Santos et al. (2009) also recognized a probable XX/XY sex system in the cytotype F but in this case, the chromosomal pair involved is the largest metacentric pair 1.

Based on the cytogenetic markers analyzed herein the karyotype of H. misionera from Amazonas River is closer related to the karyomorph C of its congener H. malabaricus. This karyomorph has been recorded in H. malabaricus populations from eastern and central portions of Amazon basin (Bertollo et al., 2000; Santos et al., 2016) and distributes southward to Paraná and Paraguay Basins reaching the northeast Argentina in the region of Misiones Province (Lopes, Fennochio, 1994) where is the type locality of H. misionera (Rosso et al., 2016). Therefore, there is a possibility that the karyomorph C remains conserved throughout the species distribution range. However, we recommend treating this assumption cautiously because karyotypic macrostructure can be a homoplastic character and could lead to mistaken inferences. Indeed, our results do not support the hypothesis that H. misionera populations from Argentina must be characterized as cytotype A, as proposed by Jacobina et al. (2018) from a geographic distribution interpretation. The sympatry of karyomorphs A and C in the northeast Argentina has been already reported (Lopes, Fennochio, 1994). All these aspects highlight the need for conducting cytogenetic studies only on well-defined taxonomic species, if we wish to improve our knowledge about the relationships between taxonomic and karyotype diversity. Further investigations are also needed to find out other aspects of the cytogenomic patterns of H. misionera populations from the Amazon and La Plata basins.

The geographic range of H. misionera is widely expanded northerly from the original localities included in the species description. New occurrences reported for the Amazon Basin are situated 2700 km northwards from the northernmost location previously known for H. misionera and 3180 km from the type locality of the species (Rosso et al., 2016). The disjunct distribution of H. misionera in the Amazon and Paraná-Paraguay systems confirms a biogeographic condition formerly suggested by Cardoso et al. (2018) grounded only in molecular data. Fish fauna shared between the Amazon and Paraguay rivers has been explained as the result of biotic dispersal events across wetlands connecting the headwaters of neighboring drainages (see Lundberg et al., 1998; Carvalho, Albert, 2011; Ota et al., 2014). The Paraguay River Basin has approximately 309 species (Britski et al., 2007; Ferreira et al., 2017), with about one-third shared with the Amazon basin (see Carvalho, Albert, 2011; Dagosta, De Pinna, 2019). Clearly, given the vastness of the Amazon basin, this region might still harbor large extensions of underexplored river systems hindering species diversity (Jézéquel et al., 2020). In this scenario, the occurrence of other populations of H. misionera should not be ruled out. Further studies focusing on biogeographic and integrative scopes may fill these sampling gaps and assess the morphological and genetic trait variation of H. misionera populations throughout the entire species distribution range.

Comparative material examined. Hoplias misionera: Argentina: Holotype: Misiones province, stream tributary of the Acaraguá River: UNMDP 574, 1, 164 mm SL. Paratypes: same locality as holotype: UNMDP 3320, 1, 174 mm SL; UNMDP 3391, 1, 149 mm SL. Formosa province, riacho Saladillo: UNMDP 3321, 1, 142 mm SL; UNMDP 3322, 1, 148 mm SL; riacho Salado: UNMDP 3327, 1, 171 mm SL; UNMDP 3328, 1, 146 mm SL; UNMDP 3329, 1, 134 mm SL; riacho Mbiguá: UNMDP 3371, 1, 154 mm SL; UNMDP 3376, 1, 165 mm SL.

Acknowledgments​

We thank to the LGBIO former students Jennefer Alves, Eugerlane Queiroz, Diego Marques, Vinícius Oliveira and Fabíola Santos that helped us with fieldwork and chromosome preparations. Dr. Eliana Feldberg (INPA) helped us with FISH technique. We are grateful to Tauanny Lima (UEMA) for providing the illustrations to Figs. 2‒3. This paper is part of the Master dissertation entitled “Estudos de taxonomia integrativa do complexo Hoplias malabaricus (Bloch, 1794) na Bacia Amazônica e drenagens adjacentes”, presented in August 2020 at the Universidade Federal do Oeste do Pará. The first author thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) by the master scholarship (88882.457158/2019‒01) and Universidade Federal do Oeste do Pará (ARNI/PROPPIT) for providing the financial support for the trip to Argentina (process 23204.013496/2019‒17), LRRR was granted by CNPQ/FAPEAM-INCT ADAPTA II (Process 465540/2014‒7; Process 062.1187/2017).

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Authors

Karen L. A. Guimarães^{1, 2} , Juan J. Rosso^{3, 4}  Mendelsohn F. B. Souza² Juan M. Díaz de Astarloa^{3, 4}  andLuís R. R. Rodrigues^{1, 2} 

^[1]    Programa de Pós-graduação em Recursos Naturais da Amazônia (PPGRNA), Universidade Federal do Oeste do Pará, Rua Vera Paz, s/n, Unidade Tapajós, Bairro Salé, 68040-255 Santarém, PA, Brazil. (KLAG) karen.guimaraes.bio@gmail.com (corresponding author); (LRRR) luisreginaldo.ufpa@hotmail.com.

^[2]    Laboratório de Genética & Biodiversidade (LGBio), Universidade Federal do Oeste do Pará, Rua Vera Paz, s/n (Unidade Tapajós), Bairro Salé, 68040-255 Santarém, PA, Brazil. (MFBS) mendelsohn2008@gmail.com.

^[3]  Grupo de Biotaxonomía Morfológica y Molecular de Peces, Instituto de Investigaciones Marinas y Costeras (IIMyC), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3350, 7600 Mar del Plata, Argentina.
(JJR) plurosso@yahoo.com.ar; (JMDA) astarloa@mdp.edu.ar.

^[4]    Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.

Authors Contribution

Karen L. A. Guimarães: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing-original draft, Writing-review and editing.

Juan J. Rosso: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Supervision, Validation, Visualization, Writing-review and editing.

Mendelsohn F. B. Souza: Methodology, Software, Validation, Visualization, Writing-review and editing.

Juan M. Díaz de Astarloa: Investigation, Resources, Supervision, Validation, Visualization, Writing-review and editing.

Luís R. R. Rodrigues: Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing-review and editing.

Ethical Statement​

The Brazilian government System of Authorization and Information in Biodiversity (SISBIO) provided the permits for fish collections (SISBIO 32653–3). The animals were anesthetized and euthanized through immersion in water containing Eugenol solution, following a procedure approved by the Animal Use Ethics Committee (CEUA) of the Universidade Federal do Oeste do Pará (CEUA/UFOPA Nº 09003).

Competing Interests

The authors declare no competing interests.

How to cite this article

Guimarães KLA, Rosso JJ, Souza MFB, Díaz de Astarloa JM, Rodrigues LRR. Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations. Neotrop Ichthyol. 2021; 19(2):e200110. https://doi.org/10.1590/1982-0224-2020-0110

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